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Test Code ANIDE Organism Referred for Identification, Anaerobic Bacteria

Biohazard Infectious

Reporting Name

Organism Ref for ID, Anaerobic Bact

Useful For

Identification of anaerobic bacteria involved in human infections

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Advisory Information


If susceptibility testing is needed; order MMLSA / Antimicrobial Susceptibility, Anaerobic Bacteria, MIC also. If susceptibilities are not appropriate, MMLSA will be canceled at report time.



Shipping Instructions


See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.



Necessary Information


1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.



Specimen Required


Supplies:

Anaerobic transport tube (T588)

Thioglycollate broth or any other suitable anaerobic transport system

Large infectious container (T146)

Specimen Type: Pure culture of organism from a source not normally colonized by anaerobes

Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds

Container/Tube:

Preferred: Anaerobic transport tube (T588)

Acceptable: Thioglycollate broth or any other suitable anaerobic transport system

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

3. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.


Specimen Minimum Volume

NA

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Identification of organism

Day(s) and Time(s) Performed

Monday through Sunday

CPT Code Information

87076-Organism ref for ID, anaerobic bact

87076-Id MALDI-TOF mass spec anaerobe (if appropriate)

87076-Anaerobe Ident (if appropriate)

87153-Anaerobe ident by sequencing (if appropriate)

87077-Identification commercial kit (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria identification (if appropriate)

87077-Additional identification procedure (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87147 x 3-Serologic agglut method 1 ident (if appropriate)

87147-Serologic agglut method 2 ident (if appropriate)

87147 x 4-Serologic agglut method 3 ident (if appropriate)

87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)

87153-Aerobe ident by sequencing (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANIDE Organism Ref for ID, Anaerobic Bact 20878-5

 

Result ID Test Result Name Result LOINC Value
ANIDE Organism Ref for ID, Anaerobic Bact 20878-5

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALA Id MALDI-TOF Mass Spec Anaerobe No, (Bill Only) No
ANAID Anaerobe Ident No, (Bill Only) No
ISAN Anaerobe Ident by Sequencing No, (Bill Only) No
COMM Identification Commercial Kit No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
STAP Identification Staphylococcus No, (Bill Only) No
STRP Identification Streptococcus No, (Bill Only) No
SIDC Ident Serologic Agglut Method 4 No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged. All bacterial organisms submitted will be identified and billed as appropriate.

Clinical Information

Anaerobic bacteria are the greatest component of the human body's normal bacterial flora colonizing the skin, oral cavity, and genitourinary and lower gastrointestinal tracts. Their presence is important in promoting vitamin and other nutrient absorption and in preventing infection with pathogenic bacteria.

 

Anaerobes generally are of low pathogenicity, but may possess virulence factors such as endotoxin or polysaccharide capsules or produce extracellular toxins. Disease occurs when a large inoculum develops in an area lacking oxygen or with a poor blood supply.

 

Typical anaerobic infections include peritonitis, abdominal or pelvic abscesses, endometritis, pelvic inflammatory disease, aspiration pneumonia, empyema, lung abscesses, sinusitis, brain abscesses, gas gangrene, and other soft tissue infections. Many Bacteroides produce beta-lactamase and are resistant to penicillins and cephalosporins. Imipenem, metronidazole, and clindamycin are effective agents, although resistance to clindamycin is increasing.

Interpretation

Isolation of anaerobes in significant numbers from well-collected specimens from blood, other normally sterile body fluids, or closed collections of purulent fluid indicates infection with the identified organism.

Clinical Reference

1. Jousimies-Somer HR, Summanen P, Citron DM, et al: Wadsworth Anaerobic Bacteriology Manual. Sixth edition. Belmont, CA, Star Publishing Co. 2002

2. Anaerobic Bacteria. Chapters 50-54. In Manual of Clinical Microbiology. 11th edition. Edited by JH Jorgensen, MA Pfaller. Washington, DC, ASM Press, 2015

3. Hall GS: Section 4. Anaerobic bacteriology. In Clinical Microbiology Procedures Handbook. Vol 1. Third edition. Edited by LS Garcia. Washington, DC, ASM Press, 2010

Analytic Time

8 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Agar plate

Method Name

Dependent on organism submitted, 1 or more of the following methods will be used: media, aero tolerance testing, conventional biochemical tests (MALDI-TOF) mass spectrometry or 16S RNA gene sequencing

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).