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Test Code DBA4 Hairy Cell Leukemia (DBA44) Immunostain, Technical Component Only


Shipping Instructions


Attach the green pathology address label and the pink Immunostains Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25 x 75 x 1 mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html


Secondary ID

70419

Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

Hairy Cell (DBA44) IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue

Cytology smears

Nonformalin fixed tissue

Nonparaffin embedded tissue

Noncharged slides

ProbeOn slides

Clinical Information

Hairy cell leukemia (DBA.44) antibody recognizes an unknown, fixation-resistant antigen that is expressed in normal mantle zone B-cells. It may be expressed in endothelial cells, monocytoid B cells, and scattered immunoblasts as well. It is characteristically expressed in hairy cell leukemia, as well as a subset of marginal zone lymphomas, and may be useful is classification of these lymphomas.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Dunphy CH: Reaction patterns of TRAP and DBA.44 in hairy cell leukemia, hairy cell variant, and nodal and extranodal marginal zone B-cell lymphomas. Appl Immunohistochem Mol Morphol 2008;16(2):135-139

2. Hoyer JD, Li CY, Yam LT, et al: Immunohistochemical demonstration of acid phosphatase isoenzyme 5 (tartrate-resistant) in paraffin sections of hairy cell leukemia and other hematologic disorders. Am J Clin Pathol 1997;108(3):3-8-315

3. Jones G, Parry-Jones N, Wilkins B, et al: Revised guidelines for the diagnosis and malignant of hairy cell leukaemia and hairy cell leukemia variant. British Journal of Haematology 2011.156:186-195

4. Sherman MJ, Hanson CA, Hoyer JD: An assessment of the usefulness of immunohistochemical stains in the diagnosis of hairy cell leukemia. Am J Clin Pathol 2011;136:390-399

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Result ID Test Result Name Result LOINC Value
70732 Hairy Cell (DBA44) IHC, Tech Only No LOINC Needed