Sign in →

Test Code ENTP Enterovirus, Molecular Detection, PCR, Plasma

Reporting Name

Enterovirus PCR, P

Useful For

Aids in diagnosing enterovirus infections

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Plasma EDTA


Specimen Required


Submit a raw clinical sample (not a culture isolate) for enterovirus PCR. This test will detect enterovirus, but will not differentiate viruses in this family or provide serotyping information.

 

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Screw-capped, sterile container

Specimen Volume: 1 mL

Collection Instructions: Spin down promptly.

Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Sunday; Varies

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87498

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ENTP Enterovirus PCR, P 29591-5

 

Result ID Test Result Name Result LOINC Value
56068 Enterovirus PCR, P 29591-5

Clinical Information

Enteroviruses are positive-sense RNA viruses in the Picornaviridae family. These viruses were initially classified by serotype as polioviruses (3 types), echoviruses (31 types, including types 22 and 23, which are now classified as parechoviruses), coxsackievirus A (23 types), and coxsackievirus B (6 types). However, genomic studies have demonstrated that there is significant overlap in the biological characteristics of different serotypes and, more recently, isolated enteroviruses are now named with consecutive numbers (eg, EV68, EV69).

 

The normal site of enterovirus replication is the gastrointestinal tract where the infection is typically subclinical. However, in a proportion of cases, the virus spreads to other organs, causing systemic manifestations, including mild respiratory disease (eg, common cold); conjunctivitis; hand, foot, and mouth disease; aseptic meningitis; myocarditis; and acute flaccid paralysis. Collectively, enteroviruses are the most common cause of upper respiratory tract disease in children. In addition, the enteroviruses are the most common cause of central nervous system (CNS) disease; they account for almost all viruses recovered in culture from spinal fluid. Differentiation of enteroviruses from other viruses and bacteria that cause CNS disease is important for the appropriate medical management of these patients.

 

Traditional cell culture methods require 6 days, on average, for enterovirus detection. In comparison, real-time PCR allows same-day detection. Detection of enterovirus nucleic acid by PCR is also the most sensitive diagnostic method for the diagnosis of CNS infection caused by these viruses.

Interpretation

A positive result indicates the presence of enterovirus RNA in the specimen.

Clinical Reference

1. Enterovirus surveillance-United States, 1970-2005. MMWR Morb Mortal Wkly Rep Sept 15 2006;55(SS08):1-20

2. Foray S, Pailloud F, Thouvenot D, et al: Evaluation of combining upper respiratory tract swab samples with cerebrospinal fluid examination for the diagnosis of enteroviral meningitis in children. J Med Virology 1999;57(2):193-197

3. Furione M, Zavattoni M, Gatti M, et al: Rapid detection of enteroviral RNA in cerebrospinal fluid (CSF) from patients with aseptic meningitis by reverse transcription-nested polymerase chain reaction. New Microbiol 1998;21(4):343-351

Analytic Time

Same day/1 day

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

Method Name

Real-Time Polymerase Chain Reaction (PCR)/RNA Probe Hybridization

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)