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Test Code EXHD Hematologic Disorders, DNA Extract and Hold

Secondary ID

64779

Useful For

Reserving nucleic acid on any specimen for which molecular analysis requiring DNA may be necessary at a future date, ensuring that adequate material for testing is available

Testing Algorithm

DNA will be extracted from the specimen and stored at -80° Celsius for 1 year from the date of the extraction process was performed. A report of "Performed" will be sent and a $50.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratory's test menu, call 800-533-1710. Additional charges will be assessed for testing performed.

Method Name

Nucleic Acid Extraction

Reporting Name

DNA Extract and Hold

Specimen Type

Varies


Specimen Required


Specimen must arrive within 168 hours of draw. Draw and package specimen as close to shipping time as possible.

 

Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.


Specimen Minimum Volume

Blood, Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred) 7 days
  Refrigerated  7 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

Paraffin-embedded material, fluids, heparinized samples

Clinical Information

It is frequently useful to obtain nucleic acid from clinical samples containing a hematopoietic neoplasm at the time of diagnosis, so that appropriate material is available for molecular analysis should subsequent testing be required. For example, when a diagnosis of acute myelogenous leukemia is made, there is a delay before karyotype information, which determines whether testing for molecular prognostic markers is necessary, is available. After this delay, the diagnostic sample is usually no longer available or the nucleic acid has degraded to such an extent that it is no longer adequate for testing. Thus, it is useful to obtain nucleic acid on such specimens promptly at diagnosis and retain it until it is known whether additional testing is necessary.

Reference Values

Not applicable

Interpretation

A report of "Performed" will be sent and a $50 processing fee will be assessed. No interpretation will be given. Should the sample be used in future testing, interpretation would be incorporated with the final testing.

Day(s) and Time(s) Performed

Monday through Saturday

Specimens received Sunday will be processed Monday.

Analytic Time

2 days

Performing Laboratory

Mayo Medical Laboratories in Rochester

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EXHD DNA Extract and Hold In Process

 

Result ID Test Result Name Result LOINC Value
MP034 Specimen Type 31208-2
41995 DNA Extract and Hold Result No LOINC Needed
38305 DNA Extraction In Process

Test Classification

N/A

Forms

1. Hematopathology Patient Information (T676) in Special Instructions.

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)