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Test Code GFAP Glial Fibrillary Acidic Protein (GFAP) Immunostain, Technical Component Only


Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html


Secondary ID

70443

Useful For

Classification of glial tumors

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

GFAP IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides

Clinical Information

Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52 kD found in glial cells, astrocytes, and ependymal cells. Immunoperoxidase staining for GFAP produces intense cytoplasmic staining of astrocytes, glial cells, and ependymal cells in normal brain. In neoplastic tissues, GFAP is useful for the identification of glial tumors such as astrocytomas and ependymomas.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Halliday GM, Cullen KM, Kril JJ, et al: Glial fibrillary acidic protein (GFAP) immunohistochemistry in human cortex: A quantitative study using different antisera. Nuerosci Lett 1996;209(1):29-32

2. Meis JM, Ho KL, Nelson JS: Gliosarcoma: A histologic and immunohistochemical reaffirmation. Modern Pathology 1990;3(1):19-24

3. Paulus W: GFAP, Ki67 and IDH1: Perhaps the golden triad of glioma immunohistochemistry. Acta Neuropathol 2009;118:603-604

4. Santos GC, Carvalho KC, Falzoni R, et al: Glial fibrillary acidic protein in tumor types with cartilaginous differentiation. Modern Pathology 2009;22:1321-1327

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

70756

LOINC Code Information

Result ID Test Result Name Result LOINC Value
70756 GFAP IHC, Tech Only No LOINC Needed