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Test Code IDENT Organism Referred for Identification, Aerobic Bacteria

Biohazard Infectious

Reporting Name

Organism Refer for ID, Aerobic Bact

Useful For

Identification of pure isolates of aerobic bacteria

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Advisory Information


If susceptibility testing is needed; also order ZMMLS Antimicrobial Susceptibility, Aerobic Bacteria, MIC. If susceptibilities are not appropriate and will not be performed, ZMMLS will be canceled at report time.



Shipping Instructions


See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.



Necessary Information


1. Specimen source is required.

2. Isolate description is required including: Gram stain reaction, morphology, and tests performed.



Specimen Required


Supplies: Infectious Container, Large (T146)

Specimen Type: Pure culture of organism from source cultured

Container/Tube: Agar slant or other appropriate media

Specimen Volume: Entire specimen

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

3. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.


Specimen Minimum Volume

NA

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Identification of organism

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87077-Organism Referred for Identification, Aerobic Bacteria

87077-Identification Commercial Kit (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)

87147-Serologic Agglut Method 2 Ident (if appropriate)

87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)

87147 x 2-6 - Serologic Agglut Method 4 Ident (if appropriate)

87077-Identification Staphylococcus (if appropriate)

87077-Identification Streptococcus (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IDENT Organism Refer for ID, Aerobic Bact 32367-5

 

Result ID Test Result Name Result LOINC Value
IDENT Organism Refer for ID, Aerobic Bact 32367-5

Clinical Information

Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.

 

Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, MALDI-TOF mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.

Interpretation

Genus and species are reported on aerobic bacterial isolates, whenever possible.

 

Bacillus species will be reported out as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.

Clinical Reference

1. Manual of Clinical Microbiology. 11th edition. Edited by JH Jorgensen, MA Pfaller, KC Carrol, et al. Washington, DC, ASM Press, 2015

2. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition. Edited by GW Procop, DL Church, GS Hall, et al. Philadelphia, Lippincott Williams and Wilkins. 2017

Analytic Time

5 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Agar plate

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
COMM Identification Commercial Kit No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
STAP Identification Staphylococcus No, (Bill Only) No
STRP Identification Streptococcus No, (Bill Only) No
SIDC Ident Serologic Agglut Method 4 No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged. All aerobically growing bacteria submitted will be identified and billed as appropriate.

Note: Mayo Medical Laboratories recommends referring suspect select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) to your state health department or the CDC for identification confirmation or exclusion.

Method Name

Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, MALDI-TOF mass spectrometry, and 16S RNA gene sequencing.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).