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Test Code JCHAI J-Chain Immunostain, Technical Component Only


Shipping Instructions


Attach the green pathology address label and the pink Immunostains Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostains Technical Only Envelope (T693)

Preferred: 2 Unstained positively charged glass slides (25 x 75 x 1 mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of Immunohistochemistry (IHC) stains and the manual requisition form can be accessed through this website: http://www.mayomedicallaboratories.com/test-info/ihc/index.html 

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: http://www.mayomedicallaboratories.com/test-info/ihc/faq.html 


Secondary ID

7048

Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

J-Chain IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue 
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

J-chain is a small, glycopeptide of 15 kD that is structurally unrelated to heavy or light chains. It serves to structurally link the immunoglobulin components of polymeric immunoglobulins IgA and IgM, and appears to play a role in secretion of antibodies at mucosal sites. B cells in the germinal center express J-chain at an early stage of differentiation, with the expression persisting in the plasma cells destined to produce IgA or IgM.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity.  If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Roberts C, Jack F, Angus B, et al: Immunohistochemical detection of CD30 remains negative in nodular lymphocyte-predominant Hodgkin's disease using enhanced antigen retrieval. Histopathology 2002 Feb;40(2):166-170

2. Isaacson P: Immunochemical demonstration of J chain: a marker of B-cell malignancy. J Clin Pathol 1979 Aug;32(8):802-807

3. Rudigar T, Ott G, Ott MM, et al: Differential diagnosis between classic Hodgkin's lymphoma, T-cell-rich B-cell lymphoma, and paragranuloma by paraffin immunohistochemistry. Am J Surg Pathol 1998 Oct;22(10):1184-1191

4. Mestecky J, Preud'homme JL, Crago SS, et al: Presence of J chain in human lymphoid cells. Clin Exp Immunol 1980 Feb;39(2):371-385

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Result ID Test Result Name Result LOINC Value
70791 J-Chain IHC, Tech Only No LOINC Needed