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Test Code KIT KIT Immunostain, Technical Component Only


Shipping Instructions


Attach the green pathology address label and the pink Immunostains Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostains Technical Only Envelope (T693)

Preferred: 2 Unstained positively charged glass slides (25 x 75 x 1 mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of immunohistochemistry (IHC) stains and the manual requisition form can be accessed through this website: http://www.mayomedicallaboratories.com/test-info/ihc/index.html 

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: http://www.mayomedicallaboratories.com/test-info/ihc/faq.html 


Secondary ID

70485

Useful For

Aids in the identification of gastrointestinal stromal tumors

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

KIT IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue

Cytology smears

Nonformalin fixed tissue

Nonparaffin embedded tissue

Noncharged slides

ProbeOn slides

Clinical Information

KIT (CD117) membrane protein is a type III tyrosine kinase growth factor receptor for stem cell factor (SCF), also known as mast cell growth factor. It is expressed in mast cells, melanocytes, and interstitial cells of Cajal. KIT is expressed in various epithelia (breast, sweat glands and salivary glands, renal tubular cells, thyroid follicular cells), testicular and ovarian interstitial cells, neurons of the central nervous system, immature myeloid cells, and trophoblastic cells. KIT staining is useful in the diagnosis of gastrointestinal stromal tumors (GISTs), germ cell tumors, mast cell disorders and acute myeloid leukemias.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Lau SK, Weiss LM, Chu PG: D2-40 immunohistochemistry in the differential diagnosis of seminoma and embryonal carcinoma: a comparative immunohistochemical study with KIT (CD117) and CD30. Mod Pathol 2007;20:320-325

2. Miettinen M, Sobin LH, Sarloma-Rikala M: Immunohistochemical spectrum of GISTs at different sites and their differential diagnosis with a reference to CD117 (KIT). Mod Pathol 2000;13(10):1134-1142

3. Sarlomo-Rikala M, Kovatich AJ, Barusevicius A, Miettinen M: CD117: a sensitive marker for gastrointestinal stromal tumors that is more specific than CD34. Mod Pathol 1998;11(8):728-734

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Result ID Test Result Name Result LOINC Value
70797 KIT IHC, Tech Only No LOINC Needed