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Test Code LVZV Varicella-Zoster Virus, Molecular Detection, PCR

Reporting Name

Varicella-Zoster Virus PCR

Useful For

Rapid (qualitative) detection of varicella-zoster virus DNA in clinical specimens for laboratory diagnosis of disease due to this virus

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Supplies:

Culturette (BBL Culture Swab) (T092)

M5 Media (T484)

Specimen Type: Swab

Sources: Miscellaneous; dermal, eye, nasal, or throat

Container/Tube: BBL CultureSwab (T092) or multimicrobe media (M5) (T484)

Collection Instructions: Place swab into M5 media (T484) or M4 media.

 

Supplies:

Culturette (BBL Culture Swab) (T092)

M5 Media (T484)

Specimen Type: Swab

Sources: Genital; cervix, vagina, urethra, anal/rectal, or other genital sources

Container/Tube: BBL CultureSwab (T092) or multimicrobe media (M5) (T484)

Collection Instructions: Place swab into multimicrobe media (M5) (T484) or M4 media.

 

Specimen Type: Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1.5 mL

 

Supplies: M5 Media (T484)

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Container/Tube:

Preferred: Multimicrobe media (M5) (T484)

Acceptable: Sterile container with 1 to 2 mL of sterile saline

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue in multimicrobe media (M5) (T484) or a sterile container with 1 to 2 mL sterile saline.


Specimen Minimum Volume

Body Fluid, Ocular Fluid, or Spinal Fluid: 0.3 mL
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Saturday; Varies

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LVZV Varicella-Zoster Virus PCR 11483-5

 

Result ID Test Result Name Result LOINC Value
SRC70 Specimen Source 31208-2
36046 Varicella-Zoster Virus PCR 11483-5

Clinical Information

Varicella-zoster virus (VZV) causes both varicella (chickenpox) and herpes zoster (shingles). VZV produces a generalized vesicular rash on the dermis (chickenpox) in normal children, usually before 10 years of age. After primary infection with VZV, the virus persists in latent form and may emerge clinically (usually in adults 50 years of age and older) to cause a unilateral vesicular eruption, generally in a dermatomal distribution (shingles).

Interpretation

Detection of varicella-zoster virus (VZV) DNA in clinical specimens supports the clinical diagnosis of infection due to this virus.

 

VZV DNA is not detected in cerebrospinal fluid from patients without central nervous system disease caused by this virus.

 

This LightCycler PCR assay does not yield positive results with other herpesvirus gene targets (herpes simplex virus, cytomegalovirus, Epstein-Barr virus).

Clinical Reference

1. Cinque P, Bossolasco S, Vago L, et al: Varicella-zoster virus (VZV) DNA in cerebrospinal fluid of patients infected with human immunodeficiency virus: VZV disease of the central nervous system or subclinical reactivation of VZV infection? Clin Infect Dis 1997;25(3):634-639

2. Brown M, Scarborough M, Brink N, et al: Varicella zoster virus-associated neurological disease in HIV-infected patients. Int J STD AIDS 2001;12(2):79-83

3. Studahl M, Hagberg L, Rekabdar E, Bergstrom T: Herpesvirus DNA detection in cerebrospinal fluid: differences in clinical presentation between alpha-, beta-, and gamma-herpesviruses. Scand J Infect Dis 2000;32(3):237-248

4. Iten A, Chatelard P, Vuadens P, et al: Impact of cerebrospinal fluid PCR on the management of HIV-infected patients with varicella-zoster virus infection of the central nervous system. J Neurovirol 1999;5(2):172-180

Analytic Time

1 day/same day

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Calcium alginate-tipped swab, wood swab, or transport swab containing gel; formalin-fixed and/or paraffin-embedded tissues

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)