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Test Code MERKC Merkel CC (MCPyV) Immunostain, Technical Component Only


Advisory Information


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html


Secondary ID

71538

Useful For

Identification of Merkel cell polyomavirus (MCPyV) infected cells

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

Merkel CC (MCPyV) IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue

Cytology smears

Nonformalin fixed tissue

Nonparaffin embedded tissue

Noncharged slides

ProbeOn slides

Clinical Information

Merkel cell polyomavirus (MCPyV) infections are common and typically benign. In rare instances, these infections lead to neoplastic transformations known as Merkel cell carcinoma (MCC) through the expression (nuclear) of MCPyV large T-antigen. MCPyV-positive MCC have been reported to behave less aggressively than MCPyV-negative MCC.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Leroux-Kozal V, Leveque N, Brodard V, et al: Merkel cell carcinoma: histopathologic and prognostic features according to the immunohistochemical expression of Merkel cell polyomavirus large T antigen correlated with viral load. Hum Pathol 2015 Mar;46(3):443-453

2. Samimi M, Molet L, Fleury M, et al: Prognostic value of antibodies to Merkel cell polyomavirus T antigens and VP1 protein in patients with Merkel cell carcinoma. A Br J Dermatol 2016 Apr;174(4):813-822

3. Richards KF, Guastafierro A, Shuda M, et al:  Merkel cell polyomavirus T antigens promote cell proliferation and inflammatory cytokine gene expression. J Gen Virol 2015 Dec;96(12):3532-3544

4. Jung HS, Choi YL, Choi JS, et al: Detection of Merkel cell polyomavirus in Merkel cell carcinomas and small cell carcinomas by PCR and immunohistochemistry. Histol Histopathol 2011 Oct;26(10):1231-1241

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MERKC Merkel CC (MCPyV) IHC, Tech Only In Process

 

Result ID Test Result Name Result LOINC Value
71542 Merkel CC (MCPyV) IHC, Tech Only In Process