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Test Code OCT4 OCT3/4 Immunostain, Technical Component Only

Advisory Information

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website:

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website:

Secondary ID


Useful For

Aids in the identification of germ cell tumors

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

OCT3/4 IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)

Reject Due To








Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

Octamer-binding transcription factor 3/4 (OCT4), also known as POU5F1, is a transcription factor expressed by embryonal stem cells and germ cells. Staining for OCT4 can aid in the diagnosis of testicular or ovarian germ cell tumors; it is highly specific for germ cell neoplasia, especially seminomas. Alternative splicing produces 2 mRNA variants from the POU5F1 gene, originally named OCT3A and OCT3B, thus the OCT3/4 name.


The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Zhao FQ: Octomer-binding transcription factors: genomics and functions. Front Biosci 2013 Jun 1;18:1051-1071

2. Cheng L, Sung MT, Cossu-rocca P, et al: OCT4: biological functions and clinical applications as a marker of germ cell neoplasia. (Review) J Pathol 2007 Jan;211(1):1-9

3. Jung SM, Chu PH, Shiu TF, et al: Expression of OCT4 in the primary germ cell tumors and thymoma in the mediastinum. Appl Immunohistochem Mol Morphol 2006 Sep;14(3):273-275

4. Lau SK, Chang KL: OCT4: a sensitive and specific immunohistochemical marker for metastatic germ cell tumors. Adv Anat Pathol 2006 Mar;13(2):76-79

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OCT4 OCT3/4 IHC, Tech Only In Process


Result ID Test Result Name Result LOINC Value
70835 OCT3/4 IHC, Tech Only No LOINC Needed


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request Form (T763) (