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Test Code SAM Entamoeba histolytica Antibody, Serum

Reporting Name

E. Histolytica Ab, S

Useful For

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess

 

Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Frozen (preferred) 5 days
  Refrigerated  5 days

Reference Values

Expected values: negative

Day(s) and Time(s) Performed

Tuesday, Thursday; 9 a.m.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86753

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SAM E. Histolytica Ab, S 22285-1

 

Result ID Test Result Name Result LOINC Value
9049 E. Histolytica Ab, S 22285-1

Clinical Information

Amebiasis is an infection by the protozoan parasite, Entamoeba histolytica. The infection is acquired by ingestion of cysts in fecally contaminated food or water; excystation and infection occur in the large intestine.

 

After excystation, trophozoites attach to the intestinal wall and liberate extracellular enzymes that enable invasion of the mucosa and spread to other organs, especially the liver and lung where abscesses develop.

 

Amebiasis (or amebic dysentery) can cause bloody diarrhea accompanied by fever and prostration. Leukocytes, WBCs, and RBCs are found in the stools. Liver abscess can develop several weeks to months later producing hepatomegaly and fever.

 

Pathogenic (Entamoeba histolytica) and nonpathogenic (Entamoeba dispar) species of Entamoeba occur. Additionally, some of those infected with pathogenic strains are asymptomatic cyst carriers.

 

Intestinal amebiasis should be diagnosed by detected Entamoeba histolytica in stool specimens.

Interpretation

A positive result suggests current or previous infection with Entamoeba histolytica.

 

Since pathogenic and nonpathogenic species of Entamoeba cannot be differentiated microscopically, some authorities believe a positive serology indicates the presence of the pathogenic species (ie, Entamoeba histolytica).

Clinical Reference

Bruckner DA: Amebiasis. Clin Microbiol Rev 1992;5:356-369

Analytic Time

Same day/1 day

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)