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Test Code T3 T3 (Triiodothyronine), Total, Serum

Reporting Name

T3 (Triiodothyronine), Total, S

Useful For

Second-order testing for hyperthyroidism in patients with low thyroid-stimulating hormone values and normal thyroxine levels

 

Diagnosis of triiodothyronine toxicosis

Testing Algorithm

See Thyroid Function Ordering Algorithm in Special Instructions.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Additional Information: 12 hours before this blood test do not take multivitamins or dietary supplements containing biotin or vitamin B7, which are commonly found in hair, skin, and nail supplements and multivitamins.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  30 days

Reference Values

Adult (≥20 years): 80-200 ng/dL

Pediatric:

0-5 days: 73-288 ng/dL

6 days-2 months: 80-275 ng/dL

3-11 months: 86-265 ng/dL

1-5 years: 92-248 ng/dL

6-10 years: 93-231 ng/dL

11-19 years: 91-218 ng/dL

Day(s) and Time(s) Performed

Monday through Friday; 5 a.m.-12 a.m.

Saturday; 6 a.m.-6 p.m.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

84480

LOINC Code Information

Test ID Test Order Name Order LOINC Value
T3 T3 (Triiodothyronine), Total, S 3053-6

 

Result ID Test Result Name Result LOINC Value
T3 T3 (Triiodothyronine), Total, S 3053-6

Clinical Information

Thyroid hormones regulate a number of developmental, metabolic, and neural activities throughout the body. The thyroid gland synthesizes 2 hormones. The 2 main hormones secreted by the thyroid gland are thyroxine, which contains 4 atoms of iodine (T4), and triiodothyronine (T3). T3 production in the thyroid gland constitutes approximately 20% of the total T3; the rest is generated by the conversion (deiodination) of T4 to T3 is also produced by conversion (deiodination) of T4 in peripheral tissues. Circulating levels of T4 are much greater than T3 levels, but T3 is biologically the most metabolically active hormone (3-4 times more potent than T4) although its effect is briefer due to its shorter half-life compared to T4.

                                                                                         

Thyroid hormones circulate primarily bound to carrier proteins (eg, thyroid-binding globulin: TBG, prealbumin, and albumin); whereas only a small fraction circulates unbound (free). Only the free forms are metabolically active. While both T3 and T4 are bound to TBG, T3 is bound less firmly than T4. Total T3 consists of both the bound and unbound fractions.

 

In hyperthyroidism, both T4 and T3 levels are usually elevated, but in a small subset of hyperthyroid patients only T3 is elevated (T3 toxicosis).

 

In hypothyroidism T4 and T3 levels are decreased. T3 levels are frequently low in sick or hospitalized euthyroid patients.

 

See Thyroid Function Ordering Algorithm in Special Instructions.

Interpretation

Triiodothyronine (T3) values >200 ng/dL in adults or > age related cutoffs in children are consistent with hyperthyroidism or increased thyroid hormone-binding proteins.

 

Abnormal levels (high or low) of thyroid hormone-binding proteins (primarily albumin and thyroid-binding globulin) may cause abnormal T3 concentrations in euthyroid patients.

Clinical Reference

1. Hay ID, Klee GG: Linking medical needs and performance goals: clinical and laboratory perspectives on thyroid disease. Clin Chem 1993;39:1519-1524

2. Klee GG: Clinical usage recommendations and analytic performance goals for total and free triiodothyronine measurements. Clin Chem 1996;42:155-159

Analytic Time

Same day/1 day

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Method Name

Electrochemiluminescence Immunoassay