Sign in →

Test Code TBET T-Box Expressed in T Cells (TBET) Immunostain, Technical Component Only

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website:

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website:

Secondary ID


Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

T-BET IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)

Reject Due To








Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

The T-box transcription factor, TBET, is a master regulator of Th1 lymphoid development. It is expressed in other hematopoietic cells including stem cells, B cells, and natural killer cells. In normal tonsil, TBET staining is seen in scattered small interfollicular T lymphocytes, with virtually no staining in the germinal centers. It is preferentially positive in T-cell lymphomas with Th1 differentiation, B-cell lymphomas of memory B-cell origin, and both classical and nodular lymphocyte predominant Hodgkin lymphoma.


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.


The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Atayar C, Poppema S, Blokzijl T, et al: Expression of the T-cell transcription factors, GATA-3 and T-bet, in the neoplastic cells of Hodgkin lymphomas. Am J Pathol 2005 Jan;166(1):127-134

2. Dorfman DM, Hawang ES, Shahsafaei A, Glimcher LH: T-bet, a T-cell associated transcription factor, is expressed in a subset of B-cell lymphoproliferative disorders. Am J Clin Pathol 2004 Aug;122(2):292-297

3. Dorfman DM, van den Elzen P, Weng AP, et al: Differential expression of T-bet, a T-box transcription factor required for Th1 T-cell development, in peripheral T-cell lymphomas. Am J Clin Pathol 2003 Dec;120(6):866-873

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TBET T-BET IHC, Tech Only In Process


Result ID Test Result Name Result LOINC Value
70871 T-BET IHC, Tech Only No LOINC Needed