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Test Code VITB2 Riboflavin (Vitamin B2), Plasma

Secondary ID

42363

Useful For

Evaluation of persons who present the signs of ariboflavinosis

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Riboflavin (Vitamin B2), P

Specimen Type

Plasma Heparin


Shipping Instructions


Send specimen in amber vial to protect from light.



Specimen Required


Patient Preparation: Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube: Green top (heparin)

Submission Container/Tube: Amber vial (T192)

Specimen Volume: 0.5 mL

Collection Instructions:

1. Place heparin collection tube on ice, keep covered.

2. Centrifuge and aliquot to amber vial

3. Freeze immediately


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Frozen (preferred) 14 days
  Refrigerated  7 days

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross OK

Other

EDTA or serum

Clinical Information

There are 3 principle vitamin B2-active flavins found in nature riboflavin, riboflavin 5-phosphate (flavin mononucleotide: FMN), and riboflavin-5'-adenosyl-diphosphate (flavin adenosine dinucleotide: FAD). In biological tissues, FMN and FAD serve as prosthetic units for a large variety of flavoproteins, which are hydrogen carriers in oxidation-reduction processes.

 

Dietary deficiency of riboflavin (ariboflavinosis) is characterized by sore throat, cheilosis (lesions on the lips), angular stomatitis (lesions on the angles of the mouth), glossitis (fissured and magenta-colored tongue), corneal vascularization, dyssebacia (red, scaly, greasy patches on the nose, eyelids, scrotum, and labia), and normocytic, normochromic anemia. Severe riboflavin deficiency may affect the conversion of vitamin B6 to its coenzyme, as well as conversion of tryptophan to niacin.

 

Riboflavin has a low level of toxicity and no case of riboflavin toxicity in humans has been reported. The limited absorptivity of riboflavin and its ready excretion in the urine normally preclude a health problem due to increased intake of riboflavin.

Reference Values

Normal: 1-19 mcg/L

Interpretation

Low concentrations in the blood plasma are indicative of nutritional deficiency. Concentrations below 1 mcg/L are considered significantly diminished. Marginally low levels probably represent nutritional deficiency that should be corrected.

Clinical Reference

1. Russell RM, Suter PM: Vitamin and trace mineral deficiency and excess. In Harrison's Principles of Internal Medicine. 17th edition. Edited by AS Fauci, DL Kasper, E Braunwald, et al. New York, McGraw-Hill Book Company, 2008, pp 441-449

2. Ball GFM: Vitamins: their role in the human body. Oxford, Blackwell Publishing, 2004, pp 289-299

3. McCormick DB: Riboflavin. In Modern Nutrition in Health and Disease. 10th edition. Edited by ME Shils, M Shike, AC Ross, et al. Philadelphia, Lippincott Williams and Wilkins, 2006, pp 434-441

4. Hustad S, McKinley MC, McNulty H, et al: Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation. Clin Chem 2002;48:1571-1577

5. Midttun O, Hustad S, Solheim E, et al: Multianalyte quantification of vitamin B6 and B2 species in the nanomolar range in human plasma by liquid chromatography-tandem mass spectrometry. Clin Chem 2005;51:1206-1216

6. Capo-chichi CD, Gueant JL, Lefebvre E, et al: Riboflavin and riboflavin-derived cofactors in adolescent girls with anorexia nervosa. Am J Clin Nutr 1999;69:672-678

Day(s) and Time(s) Performed

Monday through Friday; Evening

Analytic Time

2 days (not reported on Saturday or Sunday)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84252

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITB2 Riboflavin (Vitamin B2), P 2924-9

 

Result ID Test Result Name Result LOINC Value
61637 Riboflavin (Vitamin B2), P 2924-9