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Test Code CRITH DNA Double-Stranded (dsDNA) Antibodies by Crithidia luciliae IFA, IgG, Serum

Reporting Name

dsDNA Ab by Crithidia IFA, IgG, S

Specimen Type

Serum


Specimen Required


Only orderable as reflex. For more information see ADNAR / DNA Double-Stranded (dsDNA) Antibodies with Reflex, IgG, Serum.


Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

Only orderable as reflex. For more information see ADNAR / DNA Double-Stranded (dsDNA) Antibodies with Reflex, IgG, Serum.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86255

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CRITH dsDNA Ab by Crithidia IFA, IgG, S 58466-4

 

Result ID Test Result Name Result LOINC Value
62925 dsDNA Ab by Crithidia IFA, IgG, S 58466-4
37268 Crithidia Interpretation 69048-7

Method Name

Only orderable as reflex. For more information see ADNAR / DNA Double-Stranded (dsDNA) Antibodies with Reflex, IgG, Serum.

Secondary ID

62925

Useful For

This assay is useful for evaluating patients with suspected or who have the diagnosis of systemic lupus erythematosus.

Clinical Information

A positive Anti-Nuclear Antibodies (ANA) result usually occurs in a number of autoimmune disorders such as systemic lupus erythematosus (SLE), mixed connective tissue disease (MCTD), rheumatoid arthritis (RA), Sjögren’s syndrome (SS), and progressive systemic sclerosis (PSS). However, the incidence of low titer ANA positives increases with age in normal individuals. High titers of autoantibodies directed against dsDNA may decrease with successful therapy and increases in acute recurrence of the disease. Also, dsDNA immune complexes play a role in the pathogenesis of SLE through the deposit of the complexes in the kidney and other tissues. For these reasons, the detection of autoantibodies directed against dsDNA is diagnostically and therapeutically helpful in patients suspected or known to have SLE.

Interpretation

Negative results are considered normal.

A positive result for dsDNA antibodies is consistent with the diagnosis of SLE.

Clinical Reference

1. Soda S, Onofri L, Iannello, G: A decision support system for Crithidia luciliae image classification. Artif Intell Med 2011: 67-74

2. Homburger H, Larsen S: Detection of specific antibodies. In Clinical Immunology: Principles and Practice. First edition. Edited by R Rich, T Fleisher, B Schwartz, W Shearer, W Strober. St. Louis, Mosby-Year Book, Inc, 1996, pp 2096-2109

3. Kavanaugh A, Tomar R, Reveille J, et al: Guidelines for use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. Arch Pathol Lab Med 2000; 124:71-81

4. Kotzin B, West S: Systemic lupus erythematosus. In Clinical Immunology Principles and Practice. Second edition. Edited by R Rich, T Fleisher, W Shearer, B Kotzin, H Schroeder Jr. Mosby, 2001, pp 60.1-60.24

5. Tan EM, Cohen AS, Fries JF, et al: The 1982 Revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25:1272-1277

6. Tran T, Pisetsky D: Detection of anti-DNA antibodies. In Manual of Molecular and Clinical Laboratory Immunology, Chapter 115. Seventh edition. Edited by B Detrick, R Hamilton, J Folds. ASM Press, Washington, DC, 2006, pp. 1027-1032

7. Package insert: Bio-Rad Kallestad Crithidia luciliae Substrate

Day(s) and Time(s) Performed

Monday through Saturday; 4 p.m.

Analytic Time

2 days

Testing Algorithm

See Connective Tissue Disease Cascade (CTDC) in Special Instructions