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Test Code DESMN Desmin Immunostain, Technical Component Only

Advisory Information

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Secondary ID


Useful For

Aids in the identification of striated and smooth muscle cells and tumors derived from this cell type

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

Desmin IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Reject Due To

Tissue/Other Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides

Clinical Information

Desmin is an intermediate filament protein in muscle cells. In normal tissues, the antibody reacts with both striated and smooth muscle cells. This labeling is confined to the Z band in skeletal and cardiac muscle giving a characteristic striated appearance. In neoplastic tissues, the antibody reacts with tumors of myogenic origin such as those arising from smooth muscle (leiomyosarcomas) and those derived from striated muscle (rhabdomyosarcomas).


This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Attanoos RL, Griffin A, Gibbs AR: The use of immunohistochemistry in distinguishing reactive from neoplastic mesothelium. A Novel use for Desmin and Comparative Evaluation with Epithelial Membrane Antigen, p53, Platelet-Derived Growth Factor Receptor, P-Glycoprotein and Bcl-2. Histopathology 2003;43(3):231-238

2. Pollock L. Rampling D, Greenwal SE, Malone M: Desmin expression in rhabdomyosarcoma: Influence of the Desmin Clone and Immunohistochemical Method. J Clin Pathol 1995;48(6):535-538

3. Rangdaeng S, Truong LD: Comparative immunohistochemical staining for desmin and muscle-specific actin. A Study of 576 Cases. Am J Clin Pathol 1991;96(1):32-45

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DESMN Desmin IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70734 Desmin IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.