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Test Code DLL3 Delta-Like 3 Protein (DLL3) (SP347), Semi-Quantitative Immunohistochemistry, Manual, Tissue

Shipping Instructions

Attach the green pathology address label included in the kit to the outside of the transport container.

Necessary Information

A pathology/diagnostic report and a brief history are required.

Specimen Required

Specimen Type: Tissue

Supplies: Pathology Packaging Kit (T554)

Collection Instructions: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, "positively charged" slides with 4-microns formalin-fixed, paraffin-embedded tissue

Additional Information: One slide will be stained with hematoxylin and eosin and returned.

Secondary ID


Useful For

Diagnosis of small cell lung carcinoma (SCLC), large cell neuroendocrine carcinoma (LCNEC), amongst other tumors

Method Name

Immunohistochemistry (IHC)

Reporting Name

DLL3(SP347)Semi-Quant IHC,Manual

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Ambient (preferred)

Reject Due To

Tissue/Other Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides

Clinical Information

Delta-like 3 protein (DLL3) is an atypical Notch ligand induced by the neuroendocrine transcription factor, ASCL-1.  DLL3 is expressed on the cell membrane and in the cytoplasm of tumor cells. 


Positivity for this test will require 50% or more of the tumor cells within a specimen to express delta-like 3 protein with an intensity ranging from 1 to 3+.

Clinical Reference

1. Saunders LR, Bankovich AJ, Anderson WC, et al: A DLL3-targeted antibody-drug conjugate eradicates high-grade pulmonary neuroendocrine tumor-initiating cells in vivo. Sci Transl Med 2015;7(302):302ra136

2. Karachaliou N, Pilotto S, Lazzari C, et al: Cellular and molecular biology of small cell lung cancer: an overview. Transl Lung Cancer Res 2016;5(1):2-15

3. Laborda J, Sausville E, Hoffman T, Notario V: dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line. J Biol Chem 1993;268(6):3817-3820

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLL3 DLL3(SP347)Semi-Quant IHC,Manual In Process


Result ID Test Result Name Result LOINC Value
603333 Interpretation 59465-5
603334 Participated in the Interpretation No LOINC Needed
603335 Report electronically signed by 19139-5
603336 Material Received 81178-6
603337 Disclaimer 62364-5
603338 Case Number 80398-1