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Test Code JCV JC Virus Detection by In Situ Hybridization

Shipping Instructions

Attach the green pathology address label included in the kit to the outside of the transport container.

Necessary Information

A pathology/diagnostic report and a brief history are required.

Specimen Required

Specimen Type: Formalin-fixed, paraffin-embedded tissue block

Supplies: Pathology Packaging Kit (T554)

Specimen Volume: Entire block


Specimen Type: Slides

Slides: 4 Unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue

Secondary ID


Method Name

In Situ Hybridization

Reporting Name

JC Virus ISH

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Ambient (preferred)

Reject Due To

Tissue/Other Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue 
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides


This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

Clinical Reference

1. Fritzsche FR, Pianca S, Gaspert A, et al: Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol 2011;20(2):105-110

2. Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol 1985;18:490-496

3. Aksamit AJ Jr: Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc 1993;68:899-910

4. Muaoz-Marmol AM, Mola G, Fernandez-Vasalo A, et al: JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol 2004;63(11):1124-1130

5. Vago L, Cinque P, Sala E, et al: JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol 1996 Jun 1;12(2):139-146

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

5 day

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


88364-If additional ISH

LOINC Code Information

Test ID Test Order Name Order LOINC Value
JCV JC Virus ISH In Process


Result ID Test Result Name Result LOINC Value
71214 Interpretation 50595-8
71215 Participated in the Interpretation No LOINC Needed
71216 Report electronically signed by 19139-5
71218 Material Received 81178-6
71596 Disclaimer 62364-5
72115 Case Number 80398-1


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.