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Test Code NKX3 NKX3.1 Immunostain, Technical Component Only


Advisory Information


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Secondary ID

606692

Useful For

Identifying tumors of prostatic origin 

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

NKX3.1 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Tissue/Other Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides

Clinical Information

Homeobox NKX3.1 (NKX3.1) is a prostate-specific protein. Expression of NKX3.1 is used in the diagnosis of metastatic adenocarcinoma or ambiguous tumors that are from the prostatic origin.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Gurel B, Ali TZ, Montgomery EA, et al: NKX3.1 as a marker of prostatic origin in metastatic tumors. Am J Surg Pathol 2010;34(8):1097-1105

2. Huang H, Guma SR, Melamed J, et al: NKX3.1 and PSMA are sensitive diagnostic markers for prostatic carcinoma in bone metastasis after decalcification of specimens. Am J Clin Exp Urol 2018;6(5):182-188

3. Gary B, Azuero R, Mohanty GS, et al: Interaction of Nkx3.1 and p27kip1 in prostate tumor initiation. Am J Pathol 2004;164(5):1607-1614

4. Mohanty SK, Smith SC, Chang E, et al: Evaluation of contemporary prostate and urothelial lineage biomarkers in a consecutive cohort of poorly differentiated bladder neck carcinomas, Am J Clin Pathol 2014;142(2):173-183 

Day(s) and Time(s) Performed

Monday through Friday 

Analytic Time

1 day

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NKX3 NKX3.1 IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
606693 NKX3.1 IHC, Tech Only Bill only; no result