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Test Code OCT2 OCT-2 Immunostain, Technical Component Only


Advisory Information


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Secondary ID

70522

Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

OCT-2 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

OCT-2 is a transcription factor that binds to the octamer motif of the immunoglobulin gene promoter, recruits the coactivator BOB.1, and activates immunoglobulin gene transcription. OCT-2 is variably expressed in germinal center B cells, weakly expressed in mantle zone B cells, and weakly to moderately expressed in plasma cells. The protein is localized to the nuclear compartment. Expression of BOB.1, OCT-2, and PU.1 transcription factors are often down regulated in classical Hodgkin lymphoma. OCT-2 is overexpressed in lymphocyte-predominant (LP) cells of nodular LP Hodgkin lymphoma. These properties can be useful in the diagnosis of lymphoma.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. McCune RC, Syrbu SE, Vasef MA, et al: Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays. Mod Pathol 2006 Jul;19(7):1010-1018

2. Loddenkemper C, Anagnostopoulos I, Hummell M, et al: Differential Eu enhancer activity and expression of BOB.1/OBF.1, Oct2, PU.1, and immunoglobulin in reactive B-cell populations, B-cell non Hodgkin lymphomas and Hodgkin lymphomas. J Pathol 2004 Jan;202(1):60-69

3. Marafioti T, Ascani S, PulfordK, et al: Expression of B-lymphocyte-associated transcription factors in human T-cell neoplasm. Am J Pathol. 2003 Mar;162(3):861-871

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Result ID Test Result Name Result LOINC Value
70834 OCT-2 IHC, Tech Only No LOINC Needed

Forms

If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request Form (T763) (http://www.mayomedicallaboratories.com/it-mmfiles/ihc-and-ish-stain-request-form-mc0767-09a.pdf).