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Test Code SF1 SF-1 Immunostain, Technical Component Only

Advisory Information

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Secondary ID


Useful For

Classification of pituitary adenomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

SF-1 IHC, Tech Only

Specimen Type


Specimen Minimum Volume


Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)

Reject Due To








Wet/frozen tissue

Cytology smears

Nonformalin fixed tissue

Nonparaffin embedded tissue

Noncharged slides

ProbeOn slides

Clinical Information

SF-1, also known as steroidogenic factor 1, is a transcription factor involved in the development of the anterior pituitary and is useful in the classification of pituitary adenomas. Expression of SF-1 is observed in gonadotropic hormone producing tumors (follicular stimulating or luteinizing hormone).

Reference Values



The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Asa SL, Puy LA, Lew AM, Sundmark VC, et al: Cell type-specific expression of the pituitary transcription activator pit-1 in the human pituitary and pituitary adenomas. J Clin Endocrinol Metab 1993;77(5):1275-1280

2. Mete O, Asa SL: Clinicopathological correlations in pituitary adenomas. Brain Pathol 2012;22:443-453

3. Mete O, Asa SL: Therapeutic implications of accurate classification of pituitary adenomas. Semin Diagn Pathol 2013 Aug;30(3):158-164

4. McDonald WC, Banerji N, McDonald KN, et al: Steroidogenic Factor 1, Pit-1, and Adrenocorticotropic Hormone: A Rational Starting Place for the Immunohistochemical Characterization of Pituitary Adenoma. Arch Pathol Lab Med 2017 Jan;141(1):104-112. DOI: 10.5858/arpa.2016-0082-OA

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SF1 SF-1 IHC, Tech Only In Process


Result ID Test Result Name Result LOINC Value
72122 SF-1 IHC, Tech Only In Process


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request Form (T763) (