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Test Code UBIQ Ubiquitin (UBIQ) Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Secondary ID

70578

Useful For

Classification of neurodegenerative diseases

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOA IHC Additional, Tech Only No No
IHTOI IHC Initial, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

Ubiquitin IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Tissue/Slides Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

Ubiquitin is a polypeptide of approximately 8.5 kD found in filamentous inclusions and cytosome-related organelles in human idiopathic neurodegenerative diseases, including Alzheimer disease, Pick disease, Lewy body dementia, and Parkinson disease. Ubiquitin is also expressed in Rosenthal fibers in astrocytomas. Ubiquitin protein complexes have also been found in primary lysosome-related granules in mature neutrophils. Ubiquitin labels the periphery of senile plaques and of neurofibrillary tangles in Alzheimer disease, Lewy bodies in Parkinson disease, and Mallory bodies in alcoholic liver disease.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Chu CT, Caruso JL, Cummings TJ, et al: Ubiquitin immunochemistry as a diagnostic aid for community pathologists evaluating patients who have dementia. Mod Pathol 2000;13(4):420-426

2. Josephs KA, Holton JL, Rossor MN, et al: Frontotemporal lobar degeneration and ubiquitin immunohistochemistry. Neuropathol Applied Neurobiol 2004;30:369-373

3. Katsuse O, Dickson DW: Ubiquitin immunohistochemistry of frontotemporal lobar degeneration differentiates cases with and without motor neuron disease. Alzheimer Dis Assoc Disord 2005;19:S37-S43

4. Lennox G, Lowe J, Landon M, et al: Diffuse Lewy body disease: correlative neuropathology using anti-ubiquitin immunocytochemistry. J Neurol, Neurosurg Psychiatry 1989;52:1236-1247

5. Mackenzie IRA, Feldman HH: Ubiquitin immunohistochemistry suggests classic motor neuron disease, motor neuron disease with dementia, and frontotemporal dementia of the motor neuron disease type represent a clincopathologic spectrum. J Neuropathol Exp Neurol 2005;64(8):730-739

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
UBIQ Ubiquitin IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
70890 Ubiquitin IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.